Inter-relationships between dolichol and sterol synthesis in mammalian cell cultures.

Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4

Sterol homeostasis is essential for the function of cellular membranes and requires feedback inhibition of HMGR, a rate-limiting enzyme of the mevalonate pathway. As HMGR acts at the beginning of the pathway, its regulation affects the synthesis of sterols and of other essential mevalonate-derived metabolites, such as ubiquinone or dolichol. Here, we describe a novel, evolutionarily conserved f...

Inhibition by 6-fluoromevalonate demonstrates that mevalonate or one of the mevalonate phosphates is necessary for lymphocyte proliferation.

The sterol synthesis inhibitor 6-fluoromevalonate (Fmev) was used to explore the role of mevalonate products in lymphocyte proliferation. Fmev blocks the synthesis of isopentenyl pyrophosphate and all more distal products in the sterol pathway. When cells were cultured in lipoprotein-deficient medium, Fmev (200 microM) completely inhibited mitogen-stimulated human lymphocyte proliferation, quan...

Participation of sterol carrier protein-2 in cholesterol metabolism.

818 Introduction Lipid transport between intracellular organelles is thought to involve carrier proteins. Several different lipid transfer proteins have been isolated which are involved in the transport of phosphatidylcholine, phosphatidylinositol, phosphatidylserine, cholesterol, gangliosides, glycolipids, dolichol and vitamin D3 [ 1-31. In addition to protein mediated transport, other process...

High-Confidence Yeast Lipid Droplet Proteome 1 High-confidence proteome analysis of yeast lipid droplets identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation

The effects of statins on the mevalonic acid pathway in recombinant yeast strains expressing human HMG-CoA reductase

BACKGROUND The yeast Saccharomyces cerevisiae can be a useful model for studying cellular mechanisms related to sterol synthesis in humans due to the high similarity of the mevalonate pathway between these organisms. This metabolic pathway plays a key role in multiple cellular processes by synthesizing sterol and nonsterol isoprenoids. Statins are well-known inhibitors of 3-hydroxy-3-methylglut...